STUDY ON THE USE OF FLOW CYTOMETRY IN PRIMULA VULGARIS

Abstract

In this study, the use of flow cytometry was investigated for determining and estimating the nuclear DNA content in Primula vulgaris, an ornamental species and important model in genetics and biodiversity studies.The biological material was represented by samples of
young leaves harvested from spontaneous populations of deciduous forests in Hunedoara County. Cytometric analysis was performed using a flow cytometer, and the data obtained were processed to evaluate the quantitative variations of nuclear DNA.The results revealed a clear distribution of nuclear populations corresponding to different phases of the cell cycle and allowed the identification of possible ploidy variations between samples. The method proved efficient for the rapid characterization of plant material, providing reproducible and relevant results for genetic and breeding studies. In conclusion, flow cytometry constitutes a valuable tool in the genomic analysis of the species Primula vulgaris, facilitating the assessment of genetic variability and contributing to conservation and selection programs.