QUANTIFICATION OF ELLAGIC AND GALLIC ACID FROM LEAVES OF WILD FRUIT SPECIES BY UHPLC CHROMATOGRAPHY

Abstract

A simple, rapid and accurate chromatographic method was developed for the quantification of ellagic and gallic acid using UHPLC chromatography. The separation of the compounds was carried out using a Hypersil Gold column (150x4.6) by gradient elution with a mobile phase consisting of 0.5% formic acid aqueous solution and methanol, with a detection of the compounds at 278 nm. The obtained results confirm the presence of phenolic compounds in the leaves of some wild fruit species, respectively gallic acid varied between 5.12 (Malus sylvestris) and 82.16 (Rubus fruticosus) mg/100g DW, and ellagic acid had values between 4.28 (Crataegus monogyna) and 936.34 (Fragaria viridis) mg/100g DW. The used protocol proved to be useful for the accurate and rapid identification and quantification of the two phenolic acids.